Total mercury and selenium levels in commercial shrimp along the Pacific coast of Mexico.

The present study analyzed the content of total mercury (THg) and selenium (Se) in the muscle of shrimp collected from local markets in the 11 Pacific coastal states of Mexico. Methylmercury (MeHg) concentration, Se:Hg ratio, health benefits value from selenium consumption (HBVSe) and the permissible weekly consumption were estimated to assess the health risk to consumers. All THg and Se concentrations were below the maximum permissible limits. All hazard quotient (HQ) values were <1, however in Hermosillo, Culiacán and Guadalajara, the Se:Hg ratio and HBVSe were <1 and negative, due to the low concentrations of Se. As a general conclusion, there is no risk nor benefit from the consumption of shrimp from the Pacific coast of Mexico due to its Hg and Se content.

Assessment of potential risks to human health associated with trace elements in three commercially important shark species captured in the Central Mexican Pacific.

Shark meat is a popular protein source worldwide. However, existing national control policies for ensuring the safety and quality of shark meat are minimal. There are concerns about the sustainability of shark populations, which bioaccumulate and biomagnify potentially toxic elements at higher rates than non-predatory fish, posing a risk to human health. In this study, the contributions of essential elements to the Recommended Dietary Allowance (RDA) and potentially toxic elements to the Reference Dose (RfD) linked to shark consumption (200 g per week) were investigated for three shark species in the Central Mexican Pacific. We estimated risks and benefits for women, children, and men. Our results suggest that the three shark species are sources of Se, Co, Cr, Cu, Fe, and Zn, but poor sources of Mn for women and men aged 19-50 years. A weekly ingestion of 200 g of shark meat exceeded the RfD-As by 4-14 times and the RfD-Pb by 1.1-1.7 times for adults, indicating a potential risk to human health. The three shark species were also significant sources of potentially toxic elements for children, including Hg, Cd, Cr, and Pb. An excessive intake of Se was observed, with levels at 8.4-10.6 and 4.3-5.3 times the RDA for children aged 1-8 and 9-13 years, respectively. Although shark meat can be a good source of essential nutrients, the lack of controls in the commercialization process indicates that it should be consumed in moderation due to the potential risks associated with excessive exposure to potentially toxic elements. This caution is particularly important for children and pregnant women, who are at higher risk of health complications from consuming contaminated food.

Total Mercury and Selenium in wild Shrimp from Coastal Lagoons of Northwest Mexico: Human Health risk Assessment.

This study analyzed total mercury (THg), and selenium (Se) in edible tissues of white shrimp (Litopenaeus vannamei), blue shrimp (L. stylirostris) and brown shrimp (F. californiensis), from three states of the Northwest of Mexico in September and October 2017. Concentrations of THg and Se in the muscle were between 0.026 and 0.829 and 0.126-1.741 µg/g dry weight (dw), respectively. Significant differences were observed among Hg concentration of Sonora and Nayarit and among Se concentration of Sinaloa and Nayarit. In addition, the health risk assessment (HQ) in the three species of shrimp was between 0.550 and 0.607. All Se:Hg molar ratios were > 1 and positive HBVSe values that showed that shrimp from Northwest of Mexico does not represent a risk to human health.

Cadmium and copper mixture effects on immunological response and susceptibility to Vibrio harveyi in white shrimp Litopenaeus vannamei.

Cadmium (Cd2+) and copper (Cu2+) are considered immunotoxic metals and their presence in combination in the aquatic environment may cause effects on shrimp species as Litopenaeus vannamei. Thus, this research evaluates the combined effects of Cd2+ and Cu2+ on shrimp inoculated with Vibrio harveyi bacteria. The experiments were performed at 96-h of exposure to sublethal concentrations of both metals. No mortality was observed in organisms exposed to the sum of Criterion of Continuous Concentration (ΣCCC) in Cd + Cu mixture and those inoculated with V. harveyi. Higher clotting times were recorded in Cd + Cu + V. harveyi treatment at higher metal concentrations. No significant differences (P > 0.05) were recorded in hemocyanin content between shrimp exposed to metals and those experimentally infected. Significantly higher (P < 0.05) total hemocyte count (THC) was recorded at 96 h exposure in the ΣCCC and 10% treatments of Cd + Cu + V. harveyi experiment. Regarding Cd + Cu + V. harveyi bioassay, the highest phenoloxidase (PO) activity was recorded in shrimp inoculated with V. harveyi (0.326 ± 0.031 PO units/mg protein) at 96-h exposure. The lowest PO activity was observed in organisms exposed to Cd + Cu + V. harveyi. Regarding superoxide dismutase (SOD) activity, shrimp exposed to higher metal concentrations at 96 h showed the lowest hemolymph activity (6.03 ± 0.62 SOD units/mL). Protein decrease was observed in organisms exposed to metal mixture. The results showed that L. vannamei could be more susceptible to V. harveyi when exposed to Cd + Cu.

Essential (Cu, Zn) and nonessential (Pb, Cd) metals in the muscle of leopard groupers (Mycteroperca rosacea) from a mining port in the Gulf of California, Mexico: human health risk assessment.

Mining activities are a current environmental issue due to heavy metal release and subsequent metal uptake by organisms. In this study, we quantified the concentrations of essential (Cu, Zn) and toxic (Cd, Pb) elements in the muscle of 248 leopard groupers, Mycteroperca rosacea, captured by spearfishing and free diving close to a mining district in the Gulf of California during 2014-2015. We analysed metals using high-resolution inductively coupled plasma source mass spectrometry (HR-ICP-MS). We analysed metal concentrations by fish size, sex, maturity, season, year and risk factor for human consumption. The results indicated common levels of essential elements (Cu: 11 ± 34.3 µg/g, Zn: 377 ± 1390 µg/g) in comparison with toxic elements (Cd: 0.06 ± 0.1 µg/g, Pb: 0.98 ± 1.5 µg/g). Cadmium was within the permissible limit of Mexican standards (0.5 µg/g), but lead content bordered its limit (1.0 µg/g). Heavy metal concentrations were comparable between males and females. Metal variations were not significantly correlated with sex, maturity, season or year (p > 0.05). The evaluation of benefits (daily mineral intake) and risks (target hazard quotients) to health indicated that these fish did not represent a risk of adverse effects to consumers within worldwide limits, while the nutritional benefits were high.

Physiological and histological effects of cadmium, lead, and combined on Artemia franciscana.

This study analyzed the effects of cadmium (Cd) and lead (Pb) on growth, sexual couples, and histological structures of Artemia franciscana exposed to individual concentrations of these metals and combined. No histological effects were observed at tissue level in digestive, respiratory, nervous, and reproductive systems (i.e., necrosis, loss of regular structure) in individual and mixed applications on A. franciscana for 20 days of exposure. No significant differences (p > 0.05) were determined in final size and growth rate among the organisms exposed to Cd and those of control. For Pb, only the final size (3.59 ± 0.59 mm) of organisms exposed to the highest concentration was significantly lower (p < 0.05) than those of the control (4.53 ± 0.34 mm) group, whereas for the combined experiment, no significant differences (p > 0.05) were observed in final size and growth rate. At all Cd concentrations, mean sexual couples were significantly lower (p < 0.05) than those of the control, as well as for Pb. For the combined experiment (8 µg/L of Cd + 8 µg/L of Pb), sexual couples were not observed, indicating synergism and negative reproduction effects. The results showed that Cd and Pb aquatic environmental regulations (as the Criterion of Continuous Concentration) proposed by the US Environmental Protection Agency (EPA) should include their interactions with other metals.

Mercury and selenium biomagnification in a coastal food web from the Gulf of California influenced by agriculture and shrimp aquaculture.

The biomagnification of Hg and Se was studied using nitrogen stable isotope analysis during four seasons in a coastal lagoon of the eastern central Gulf of California. This lagoon receives agricultural, municipal, and shrimp aquaculture effluents. The species were categorized into organism groups and presented a significant accumulation of Hg and Se with respect to the sources, while the concentration of both elements in sediment and suspended particulate matter (SPM) was low. Our data confirms the positive transfers (biomagnification factors >1) of Hg and Se in the entire studied food web, and it was structured in five trophic levels across all seasons. Additionally, there were no linear correlations between the molar Se:Hg ratios and the trophic levels of the organism groups. However, the Se:Hg ratios among organism groups were >1, which indicates that there is an excess of Se and that it is not a limiting factor for the detoxification of Hg.

Concentrations of Silver, Chrome, Manganese and Nickel in Two Stranded Whale Sharks (Rhincodon typus) from the Gulf of California.

Concentrations of Ag, Cr, Mn and Ni were measured in tissues of two whale sharks (Rhincodon typus) stranded in La Paz Bay (LAP) and Punta Bufeo (PB), Gulf of California, during 2017 and 2018. The concentration range of Ni (0.012-1.12 µg/g ww) and Cr (0.16-5.21) in the epidermis of both specimens was lower compared to the concentrations found in the epidermis of whale sharks from East Africa. The whale shark from LAP exhibited higher levels of Mn (4.45 µg/g ww), Ni (0.284 µg/g ww) and Cr (5.21 µg/g ww) in the muscle compared to another filter feeder shark, the megamouth, from Taiwan and from Brazil. The highest concentrations of Ag were found in the heart (3.70) of the individual from LAP and in the filtering pads (1.93) of the shark from PB. Chromium in all selected tissues and the Mn found in the skeletal muscle, testicles (0.50), liver (Right lobe, 1.28; Left lobe, 1.63) and gills (1.54) of both sharks exceeded the limit established by the FAO/WHO for fish products.

Variation of essential and non-essential trace elements in whale shark epidermis associated to two different feeding areas of the Gulf of California.

The Gulf of California represents an important hotspot for whale shark (Rhincodon typus) aggregation. Anthropogenic activities and natural sources could expose sharks to high levels of trace elements (TEs). To determinate these levels in this endangered species, concentrations of As, Cd, Cu, Pb and Zn (in ng/g ww) were measured in 130 whale shark skin biopsies and 44 zooplankton samples collected from two areas of the Gulf of California, Bahía de Los Angeles (BLA) and Bahía de La Paz (LAP) during 2016-2018. For biopsies, Zn exhibited highest concentrations in BLA (2016-2017, 298 ± 406; 2017-2018, 1959 ± 2545) and at LAP (in 2016-2017, 595 ± 554; in 2017-2018, 2642 ± 1261). On the other hand, Cd (BLA 2016-2017, 3 ± 3; LAP 2016-2017, 4 ± 3; BLA 2017-2018, 17 ± 14; LAP 2017-2018, 13 ± 10) and Pb (BLA 2016-2017, 7 ± 7; LAP 2016-2017, 15 ± 32; BLA 2017-2018, 69 ± 76; LAP 2017-2018, 7 ± 5) showed lowest concentrations. Significant differences in TE concentrations between sites and periods occurred. Arsenic found in shark biopsies from La Paz suggested enrichment and/or increased bioavailability in this area. Sex alone was not a significant factor in TE concentration; nevertheless, a sex-dependent difference in correlation of TE concentration and size was noted (negative in males, positive in females). This indicates feeding strategies of whale shark may be sex and size segregated. During 2017-2018, zooplankton and sharks showed enrichment in all TEs. Essential elements were not biomagnified by sharks. Lead was biomagnified through zooplankton. Strong positive correlation between selected elements indicates that Zn, Cd and Pb follow the same metabolic route in the sharks' body.

Inventory of Pb emissions from one of the largest historic Pb smelter worldwide: 118-year legacy of Pb pollution in northern Mexico.

The atmospheric Pb emissions (1901-2019), from one of the world's largest non-ferrous metallurgical complexes (Met-Mex in Torreón, México), were estimated based on historical records of modifications in the design, processes, and production volumes. Eight historical periods, with differing amounts of Pb emissions, were distinguished: (1) Essentially no controls (1901-1960); (2) migration to limited controls (1961-1972) by conversion to a Pb-Zn smelter-refining complex and installation of SO2 collectors in 1961-1963; (3) completion to limited control (1973-1977) by the installation of a third H2SO4 collector and a low-efficiency filtration system; (4) maintenance of limited control with no changes (1978-1987); (5) migration to strict control (1988 to 1998) by updating H2SO4 collectors and installation of fertilizer and SO2 liquid extraction plants; (6) completion to strict control (1999-2000) by the installation of state-art technology filtration systems and roofing working areas; (7) migration to abatement (2001-2003) by implantation of good management practices; and (8) maintenance of abatement following good management practices (2004-2019). Based on differences between those periods, we reconstructed the evolution of the Pb emission reduction efficiency (ER in %) and Pb emission factors (EF in gram/ton) for the Torreón complex. Pb emitted by the complex over the past 118 years totaled 23,350-27,580 t, with most of it (63-75%) occurring when emission controls were negligible (pre-1960 period). In comparisons with other facilities worldwide (e.g., the USA, Canada, and Europa), the modification in Met-Mex for control the Pb emission occurred several years. Emissions from the primary Pb-Zn smelter-refining are released mostly to the atmosphere from the sintering, smelting, drossing, and refining. While Pb emissions from the facility have declined by over an order of magnitude to contemporary levels (≤ 12.6 t/year), the current Pb rates still account for atmospheric Pb levels that are 2-3 times higher the USEPA standard and still constitutes a major health threat in Torreón.

210Po Activity and concentrations of selected trace elements (As, Cd, Cu, Hg, Pb, Zn) in the muscle tissue of tunas Thunnus albacares and Katsuwonus pelamis from the Eastern Pacific Ocean.

Daily mineral intake (DMI) of Cu and Zn, percentage weekly intake (PWI) of As, Cd, Hg, Pb, and doses of (210)Po were estimated by using their elemental concentration in muscle of two tuna species and the average tuna consumption in Mexico. Skipjack tuna Katsuwonus pelamis had significantly (p < 0.05) higher levels of As (1.38 µg g(-1) dw) and Cu (1.85 µg g(-1) dw) than yellowfin tuna Thunnus albacares, whereas Pb concentrations (0.18 µg g(-1) dw) were significantly (p < 0.05) higher in T. albacares. The sequence of elemental concentrations in both species was Zn > Cu > As > Hg > Pb > Cd. In T. albacares, concentrations of Cd and Pb in muscle tissue were positively correlated (p < 0.05) with weight of specimens, while Cu was negatively correlated. DMI values were below 10 %. PWI figures (<2 %) are not potentially harmful to human health. (210)Po concentration in T. albacares and K. pelamis accounts for 13.5 to 89.7 % of the median individual annual dose (7.1 µSv) from consumption of marine fish and shellfish for the world population.